Ⅰ. Recommended products

1. Genes, vectors, plasmids (6w+): including vector plasmids used by various hosts (mammalian cells, lentivirus, Escherichia coli, yeast, adeno-associated viruses, plants, insects, etc.), gene template plasmids of various species, and abundant gene overexpression/interference/knockout/transcription regulation/promoter detection plasmids, see detailshttp://www.miaolingbio.com/lists/13.html。

Bulk plasmids: 100μg of detoxified dry powder plasmids.

Plasmid libraries: CRISPR-Cas9 libraries, including human and mouse whole-genome knockout libraries and activation libraries; cDNA library plasmids, including human brain, mouse brain, and rat brain cDNA libraries.

4. Competency: including E. coli competence and Agrobacterium competence that play the role of protein expression, gene cloning, transgenesis, hybridization, etc., such as DH5a, Stbl3, etc.

5. Cell lines: normal cell lines including human and mouse, rats, hamsters and other animal cell lines, such as 293T, WS602, etc.

6. Strains: including a rich variety of bacterial strains and fungal strains, such as RN4220 Staphylococcus aureus, Acinetobacter, etc.

7. Plant seeds: including Arabidopsis thaliana mutant/wild seeds and tobacco seeds.

8. Other high-quality reagents: homologous recombination kits, plasmid extraction kits, protein antibodies, ready-to-use antibiotics, transfection reagents, infection enhancers, auxiliary phages, etc.

Ⅱ. Recommendation services

1. Plasmid vector construction: Based on customer requirements, design and construct vectors such as gene overexpression/interference/knockout/subcellular localization/hybridization/promoter detection to meet the diversified needs of customers.

2. Mass plasmid extraction: provide different levels of plasmid extraction services.

3. Cell line construction: provide gene overexpression stable monoclonal cell lines, knockout monoclonal cell lines, etc.

4. Lentiviral packaging: overexpression/interference/knockout lentivirus packaging, see http://www.miaolingbio.com/lists/17.html for details.

5. Adeno-associated virus packaging: overexpression/interference/knockout of adeno-associated virus packaging.

6. Nucleic acid synthesis: siRNA/miRNA fragment synthesis service, siRNA provides three guarantees and one package.

7. Recombinant protein expression customization service.

8. Other services: RNA extraction, reverse transcription, CRISPR-Cas9 library customization.